Basic Molecular Biology MCQ Basic Molecular Biology 1 / 20 1. Which enzyme is used to join nicks in the DNA strand? A. Primase B. DNA Polymerase C. DNA Ligase D. Endonuclease 2 / 20 2. Which enzyme is used in the unwinding of DNA? A. Ligase B. Topoisomerase C. Helicase D. Exonuclease 3 / 20 3. Which of the following base-pairing rule is correct? A. Adenine with guanine and thymine with cytosine B. DNA base pairing is non-specific C. Adenine with cytosine and guanine with thymine D. Adenine with thymine and guanine with cytosine 4 / 20 4. During DNA replication the synthesis of the __________strand of DNA results in_________ fragments A. Leading , Okazaki fragments B. Lagging , Okazaki fragments C. LaKornberg segment D. Double-helix segment 5 / 20 5. Short strands of _________primer are used in DNA replication A. DNA B. RNA C. Histone D. Protein 6 / 20 6. Which of the following regarding the basic mechanism of gene expression is correct? A. DNA ―> tRNA ―> protein B. RNA ―> protein―> mRNA C. RNA ―> protein ―> DNA D. DNA ―> mRNA ―> protein 7 / 20 7. With respect to nucleosides which of the following is paired correctly? A. Purine – Adenosine, Thymidine B. Purine – Guanosine, Thymidine C. Pyrimidine – Uridine, Cytidine D. Pyrimidine – Uridine, Adenosine 8 / 20 8. According to Chargaff’s rule the two strands of DNA has ___________ A. Same molecular weight B. Same amount of A and G C. Different amount of A and G D. Different molecular weight 9 / 20 9. In one strand of a double stranded DNA the rate of occurrence of A is 3 times C in consecutive 10 bases. So how many G will be there in 100 base pairs of a DNA duplex?[Consider G=T in one strand]. A. 30 B. 20 C. 40 D. 60 10 / 20 10. Which of the following function of DNA is necessary for the purpose of evolution? A. Replication B. Transcription C. Translation D. Mutation 11 / 20 11. DNA always carries the genetic information for all organisms. A. True B. False 12 / 20 12. What should be the complementary strand of 3’….ATGGCTTGA….5’? A. 3’….TACCGAACT….5’ B. 5’….TACCGAACT….3’ C. 3’….TAGGCAAGT….5’ D. 5’….TAGGCAAGT….3’ 13 / 20 13. It is easy to break the bond between A and T than in between G and C. A. True B. False 14 / 20 14. Check which one of the following mechanisms is wrongly paired with its location of occurrence? A. Transcription – nucleus B.Post transcriptional mechanism – ER C. Translation – cytoplasm D. Post translational mechanism – ER 15 / 20 15. Which of the following is not a part of a nucleoside? A. Deoxyribose sugar B. Glycosidic linkage C. Phosphate D. Base 16 / 20 16. Polymerization of dNTP by DNA polymerase is in 3’ → 5’ direction. A. True B. False 17 / 20 17. Which enzyme is responsible for synthesizing a complementary DNA strand during DNA replication? a) DNA ligase B. RNA polymerase C. Helicase D. DNA polymerase 18 / 20 18. Where does translation occur in a eukaryotic cell? A. Cytoplasm B. Mitochondria C. Nucleus D. Endoplasmic reticulum 19 / 20 19. During transcription, what type of RNA is synthesized from a DNA template? A. Sn RNA B. rRNA C. mRNA D. tRNA 20 / 20 20. What is the central dogma of molecular biology? A. Formation of DNA and RNA B. Structure of DNA and RNA C. Process of Protein Folding D. DNA Replication, Transcription, and Translation Your score isThe average score is 60% 0% Restart quiz